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Quantification of Cytoskeletal Dynamics in Time-Lapse Recordings
René Schneider1, Arun Sampathkumar1, Staffan Persson2
1Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
Current Protocols in Plant Biology
|May 16, 2019
Summary
This protocol standardizes microtubule dynamics analysis in plant cells using live-cell microscopy. It provides accessible tools and code to quantify cytoskeletal dynamics, aiding cell biology research.
Area of Science:
- Plant Cell Biology
- Cytoskeletal Dynamics
- Live-Cell Imaging
Background:
- The plant cytoskeleton is crucial for cell growth, division, and defense.
- Cytoskeletal organization and dynamics are highly dynamic and complex.
- Quantitative analysis of cytoskeletal dynamics is often laborious and limited.
Purpose of the Study:
- To provide a standardized, step-by-step protocol for analyzing microtubule dynamics in plant cells.
- To offer example data and post-processing code for Fiji, enabling adaptation by researchers.
- To facilitate quantitative assessment of cytoskeletal dynamics for improved understanding of cell biology.
Main Methods:
- Utilizing live-cell microscopy techniques.
- Employing genetically encoded fluorophores for visualization.
- Developing and providing a standardized analysis protocol with example data and code for Fiji.
Main Results:
- A standardized protocol for analyzing microtubule dynamics is presented.
- Example data and adaptable code for post-processing in Fiji are provided.
- The protocol aims to simplify and standardize quantitative analysis of cytoskeletal dynamics.
Conclusions:
- This protocol offers a valuable tool for researchers studying plant cell cytoskeletal dynamics.
- Standardized quantitative analysis methods are essential for advancing cell biology.
- The developed tools can be modified to suit diverse research needs in cytoskeletal analysis.
Keywords:
catastrophegrowth speedlive-cell imagingmicrotubule dynamicsmicrotubule-end labelingquantitative fluorescence microscopyrescueshrink speedMore Related Videos
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