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Updated: Jan 24, 2026

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
Published on: August 4, 2022
Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer
Kondapa Naidu Bobba1, Anupama Binoy, Seyoung Koo
1Amrita Centre for Industrial Research and Innovation, Amrita School of engineering, Amrita Vishwa Vidyapeetham, Coimbatore, 641-112, India. b_sankarprasad@cb.amrita.edu.
Abstract:
Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.
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