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Smartphone-imaged microfluidic biochip for measuring CD64 expression from whole blood
Tanmay Ghonge1, Hatice Ceylan Koydemir, Enrique Valera
1Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. rbashir@illinois.edu.
The Analyst
|May 17, 2019
Summary
A novel smartphone-based microfluidic biochip rapidly measures neutrophil CD64 (nCD64) expression for early sepsis diagnosis. This point-of-care device offers a quick, automated alternative to traditional methods, potentially improving patient outcomes.
Area of Science:
- Biomedical Engineering
- Point-of-Care Diagnostics
- Sepsis Research
Background:
- Sepsis is a leading cause of death globally, necessitating improved diagnostic tools.
- Neutrophil CD64 (nCD64) expression is a sensitive biomarker for sepsis, but current detection methods are time-consuming.
- Rapid, automated point-of-care (POC) diagnostics are crucial for timely sepsis intervention.
Purpose of the Study:
- To develop and validate a smartphone-imaged microfluidic biochip for rapid nCD64 detection.
- To assess the device's performance against flow cytometry for sepsis diagnosis.
- To demonstrate the potential of POC nCD64 measurement for enhanced patient care.
Main Methods:
- Unprocessed whole blood was used in a microfluidic device with anti-CD64 functionalized pillars.
- Neutrophils were captured, and images were analyzed using a smartphone microscope.
- A statistical model quantified nCD64 expression by measuring cell capture probability.
Main Results:
- The microfluidic biochip detected nCD64 expression in under 50 minutes.
- The device showed a strong correlation (R² = 0.82) with flow cytometry for nCD64 measurement.
- Analysis of patient samples confirmed the device's applicability and correlation with clinical sepsis status.
Conclusions:
- A smartphone-imaged microfluidic biochip provides a rapid and accurate method for nCD64 detection.
- This POC technology can significantly aid in the early diagnosis and management of sepsis.
- Widespread deployment in ICUs could improve global patient care for sepsis.
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