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Selective Enumeration of Bifidobacterium bifidum , Enterococcus faecium , and Streptomycin-Resistant Lactobacillus
Melissa L Calicchia1, Connie I E Wang1, Takehiro Nomura1
1Silliker Laboratories of California, Inc., 1139 E. Dominguez Street, Carson, California 90746.
Abstract:
Modified VF-Bouillon agar with 0.5 mg/ml lithium chloride, 20 μg/ml sodium lauryl sulfate, 5 mg/ml sodium propionate, and 10 μg/ml neomycin sulfate was used with a triple-layer diffusion technique to selectively enumerate Bifidobacterium bifidum . Modified Brigg's agar was used to enumerate Enterococcus faecium . Modified Brigg's agar with 1,200 μg/ml streptomycin sulfate was used in a double-layer diffusion technique to selectively enumerate a streptomycin-resistant strain of Lactobacillus acidophilus . Selective enumeration of the individual bacterial components was compared to the mixture with an average 99% recovery of each component.
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