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Complement proteins regulating macrophage polarisation on biomaterials.

N Araújo-Gomes1, F Romero-Gavilán2, Y Zhang3

  • 1Departamento de Ingeniería de Sistemas Industriales y Diseño, Universitat Jaume I, Av. Vicent-Sos Baynat s/n, Castellón 12071, Spain; Department of Medicine, Universitat Jaume I, Av. Vicent-Sos Baynat s/n, Castellón 12071, Spain.

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|May 26, 2019
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Summary
This summary is machine-generated.

Higher concentrations of (3-glycidoxypropyl)-trimethoxysilane) (GPTMS) on titanium implants increase inflammatory responses and reduce osseointegration by promoting complement protein adsorption and macrophage polarization.

Keywords:
Complement systemDental implantsHybrid sol-gelImmune responseMacrophage plasticityProteomics

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Area of Science:

  • Biomaterials Science
  • Immunology
  • Surface Chemistry

Background:

  • Biomaterial implantation triggers protein deposition, influencing cellular responses and potentially causing foreign body reactions via complement activation.
  • Understanding surface modifications is crucial for controlling biological responses to implants.

Purpose of the Study:

  • To investigate the effect of varying (3-glycidoxypropyl)-trimethoxysilane (GPTMS) concentrations on sol-gel coatings for titanium implants.
  • To evaluate the impact of these coatings on inflammatory responses, complement protein deposition, and osseointegration.

Main Methods:

  • Synthesis of sol-gel coatings on titanium implants using different concentrations of GPTMS.
  • In vitro assessment of macrophage (RAW 264.7) inflammatory cytokine secretion (TNF-α, IL-10).
  • In vivo implantation in rabbit tibia to evaluate osseointegration.
  • Analysis of complement protein (CRP, FCN2) deposition on implant surfaces.

Main Results:

  • Increased GPTMS surface concentrations led to higher secretion of TNF-α and IL-10 by macrophages.
  • Osseointegration in rabbit tibia decreased with higher GPTMS concentrations.
  • Elevated deposition of complement proteins, C-reactive protein (CRP) and ficolin-2 (FCN2), was observed with higher GPTMS concentrations.
  • Complement protein adsorption correlated with increased inflammatory potential and conditioned macrophage polarization.

Conclusions:

  • The inflammatory potential of GPTMS-functionalized titanium implants is concentration-dependent.
  • Higher GPTMS concentrations enhance complement protein adsorption, leading to increased inflammation and impaired osseointegration.
  • Surface modification with GPTMS can modulate macrophage behavior through complement protein interactions.