Related Experiment Video
Updated: Jan 24, 2026

Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
Antibacterial Activity of the Glucose Oxidase/Glucose System in Liquid Whole Egg
D Dobbenie1, M Uyttendaele1, J Debevere1
1Department of Food Technology and Nutrition, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, Coupure Links 653, B-9000 Ghent, Belgium.
Abstract:
The effect of glucose oxidase (GOX) in different GOX/glucose combinations on four commonly occurring microorganisms in liquid whole egg was evaluated. The addition of a combination of 500 U GOX and 0.5 g glucose in 100 ml liquid whole egg killed Salmonella enteritidis , Micrococcus luteus and Bacillus cereus inoculated at 103cells/ml after 5 days of storage at 7°C, and showed a bacteriostatic activity on Pseudomonas fluorescens . The latter possesses the strongest catalase activity of the four bacteria tested, enabling it to more rapidly break down the hydrogen peroxide produced enzymatically by the GOX/glucose system. The efficacy of the above-mentioned combination was tested on the natural contamination flora of liquid egg and compared to the effect of a simple β-d-glucono-δ-lactone (GDL) treatment (0.7 g/100 ml). The GOX/glucose combination prolonged the lag phase by 6 days. Subsequently, the bacterial flora started to develop, but growth remained retarded by 2 log units. Since GDL did not exert antibacterial activity, growth inhibition by the GOX/glucose system cannot be ascribed to a pH decrease as a result of the production of gluconic acid in the system.
More Related Videos
10:35Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
Published on: April 6, 2022
06:28A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood
Published on: August 12, 2016
Related Concept Videos
Glucose Homeostasis: Regulation of Blood Glucose
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Glucose Absorption Into the Small Intestine
Hormones Regulating Blood Glucose
In addition to accelerating glucose uptake and utilization, insulin has...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
Insulin and C-peptide are...
Secondary Active Transport