The effects of conditioning startling acoustic stimulation (SAS) on the corticospinal motor system: a SAS-TMS study

A startling acoustic stimulus (SAS) could cause transient effects on the primary motor cortex and its descending tracts after habituation of reflex responses. In the literature, there is evidence that the effects of SAS depend on the status of M1 excitability and delivery time of SAS. In this study, we aimed to comprehensively investigate the effects of SAS on the excitability of primary motor cortex. Eleven healthy subjects participated in this study. Transcranial magnetic stimulation (TMS) was delivered to the hot spot for left biceps at rest and during isometric right elbow flexion (10, 30, and 60% of their maximum voluntary contraction, MVC). There were three SAS conditions: (1) No SAS; (2) SAS was delivered 50 ms prior to TMS (SAS50); (3) SAS 90 ms prior to TMS (SAS90). For each subject, the induced MEP amplitude was normalized to the largest response at rest with No SAS. Two-way ANOVAs (4 force levels × 3 SAS conditions) with repeated measures were used to determine the differences under different conditions. For the MEP amplitude, there were significant force level effect and FORCE LEVEL × SAS interactions. Specifically, the MEP amplitude increased with force level. Furthermore, post hoc analysis showed that the MEP amplitude reduced during SAS50 and SAS90 compared to No SAS only at rest. Our results provide evidence that a conditioning SAS causes a transient suppression of the corticospinal excitability at rest when it is delivered 50 ms and 90 ms prior to TMS. However, a conditioning SAS has no effect when the corticospinal excitability is already elevated with an external visual target.