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CTRP13 attenuates vascular calcification by regulating Runx2.

Yongxia Li1, Wenzhe Wang1, Yuelin Chao2

  • 1Department of Nephrology, The Central Hospital of Wuhan, Huazhong University of Science and Technology, Wuhan, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|May 31, 2019
PubMed
Summary
This summary is machine-generated.

C1q/TNF-related protein-13 (CTRP13) protects against vascular calcification by downregulating Runx2 expression. Lower CTRP13 levels in chronic renal failure patients and rats correlate with increased arterial calcification, suggesting CTRP13 as a therapeutic target.

Keywords:
chronic renal failuremRNA decayphenotypic switchvascular smooth muscle cells

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Area of Science:

  • Cardiovascular Biology
  • Endocrinology
  • Nephrology

Background:

  • Vascular calcification is a significant contributor to cardiovascular disease.
  • C1q/TNF-related protein-13 (CTRP13) is an adipokine with known cardiovascular roles, but its function in vascular calcification is unknown.

Purpose of the Study:

  • To investigate the role of CTRP13 in the development of vascular calcification.
  • To explore the underlying molecular mechanisms of CTRP13's action in vascular calcification.

Main Methods:

  • Collected blood samples from chronic renal failure (CRF) patients and adenine-induced CRF rats.
  • Administered ectopic CTRP13 to CRF rats and treated arterial rings and vascular smooth muscle cells (VSMCs) with CTRP13.
  • Utilized β-glycerophosphate to induce calcification in VSMCs and arterial rings.
  • Investigated the effect of CTRP13 on Runx2 expression and its upstream regulators, including tristetraprolin (TTP).

Main Results:

  • Serum CTRP13 levels were decreased in CRF patients and rats and negatively correlated with aortic calcification.
  • CTRP13 treatment attenuated calcium accumulation and alkaline phosphatase activity in CRF rat aortas.
  • CTRP13 inhibited VSMC osteogenic transition and reduced calcification, an effect dependent on Runx2.
  • CTRP13 repressed TTP phosphorylation, activating TTP and leading to Runx2 mRNA destabilization.

Conclusions:

  • CTRP13 plays a protective role against vascular calcification.
  • CTRP13 attenuates vascular calcification by inhibiting VSMC osteogenic transition via the TTP-Runx2 pathway.
  • CTRP13 represents a potential therapeutic target for preventing vascular calcification in CRF.