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Published on: March 9, 2021
Rhodococcus and Yarrowia-Based Lipid Production Using Lignin-Containing Industrial Residues
Rosemary K Le1, Kristina M Mahan1, Arthur J Ragauskas2,3,4
1Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, USA.
Abstract:
Improvement in biorefining technologies coupled with development of novel fermentation strategies and analysis will be paramount in establishing supplementary and sustainable biofuel pathways. Oleaginous microorganisms that are capable of accumulating triacylglycerides (TAGs) and fatty acid methyl esters (FAMEs), such as Rhodococcus and Yarrowia species, can be used to produce second-generation biofuels from non-food competing carbon sources. These "microbiorefineries" provide a pathway to upgrade agricultural and industrial waste streams to fungible fuels or precursors to chemicals and materials. Here we provide a general overview on cultivating Rhodococcus and Yarrowia on agro-waste/industrial biomass pretreatment waste streams to produce single-cell oils/lipids and preparing samples for FAME detection.
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