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Producing Oleaginous Microorganisms Using Wastewater: Methods and Guidelines for Lab- and Industrial-Scale Production
Kayla M Rude1,2, Tyler J Barzee3, Annaliese K Franz4,5
1Department of Chemistry, University of California, Davis, Davis, CA, USA.
Abstract:
Cultivation of oleaginous microorganisms on wastewater provides alternative biofuel options while also acting as a remediation technique for alternative wastewater treatment. This chapter describes guidelines and methods for the production of oleaginous microorganisms-with a focus on microalgae-using wastewater as a growth medium while considering a variety of general challenges for both lab- and industrial-scale production. Cultivation techniques described here range in scale from microplates with 10-mL working volumes, up to multigallon, industrial-scale microorganism cultivation, with a focus on microalgae. This chapter includes guidelines for the preparation of wastewater and selection of oleaginous microorganisms combined with methods for the production of oleaginous microorganisms cultivated using wastewater.
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