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Updated: Jan 24, 2026

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay
Published on: April 28, 2014
Assay Procedures for Commercial Probiotic Cultures
Tim McCann1, Trish Egan2, George H Weber1
1Georgetown Technology Group, 11240 S.W. Lynnvale Dr., Portland, Oregon 97225, USA.
Abstract:
A number of commercially available cultures, marked for human consumption, were analyzed for viability using a resuspension medium consisting of KH2PO4, Na2HPO4, cysteine, Tween 80, agar, and an antifoam agent together with modifications of multiple-layer diffusion techniques. These methods were compared to the use of MRS plus cysteine, acidified MRS, and M17 culture media and found to be superior in the selection and enumeration of dried cultures of mixed genera.
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