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Thrombin-induced platelet aggregation -effect of dabigatran using automated platelet aggregometry
Mie Shimizu1,2, Tatsunori Natori1, Keisuke Tsuda1
1Division of Neurology and Gerontology, Department of Internal Medicine, School of Medicine, Iwate Medical University, Morioka, Japan.
Platelets
|June 5, 2019
Summary
Dabigatran, a direct oral thrombin inhibitor, effectively reduces thrombin-induced platelet aggregation. This study developed a new method to assess dabigatran's antiplatelet activity in vitro.
Area of Science:
- Pharmacology
- Hematology
- Biochemistry
Background:
- Dabigatran exhibits both anticoagulant and antiplatelet effects.
- Evaluating dabigatran's impact on thrombin-induced platelet aggregation is challenging due to clot formation.
- A novel method is needed to accurately assess this antiplatelet activity.
Purpose of the Study:
- To establish a new method for measuring thrombin-induced platelet aggregation.
- To investigate the in vitro antiplatelet effects of dabigatran on thrombin-induced platelet aggregation.
Main Methods:
- Automated light transmission aggregometry (CS2400) was used with platelet-rich plasma (PRP) from 40 healthy subjects.
- Thrombin-induced fibrin polymerization was inhibited using Gly-Pro-Arg-Pro (GPRP).
- Dabigatran's concentration-dependent inhibitory effects were evaluated.
Main Results:
- A standard method for thrombin-induced platelet aggregation was established.
- Dabigatran demonstrated potent, concentration-dependent inhibition of platelet aggregation.
- Half maximal inhibitory concentrations (IC50) for dabigatran were 10.5 nM and 40.4 nM against 0.5 and 1.0 U/mL thrombin, respectively.
Conclusions:
- The developed method effectively assesses thrombin-induced platelet aggregation.
- Dabigatran exhibits significant in vitro antiplatelet activity against thrombin-induced aggregation.
- This study provides a reliable method for evaluating dabigatran's antiplatelet effects.
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