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Updated: Jan 23, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
A coumarin-based fluorescent probe for hypochlorite ion detection in environmental water samples and living cells
Mingqin Shangguan1, Xingzong Jiang1, Zhen Lu1
1College of Chemical Engineering, Fuzhou University, Xueyuan Road No. 2, Fuzhou 350116, China.
Abstract:
In this study, we developed a new fluorescent probe (CMM) based on coumarin dye and malononitrile, for highly sensitive and selective detection of hypochlorite ion (ClO-). CMM showed a 45-fold fluorescence enhancement at 459 nm in the presence of ClO- and displayed an excellent selectivity over other competing species. The probe featured a fast response time (<15 s), which could be in favor of the real-time detection towards ClO-. Meanwhile, probe CMM could effectively monitor ClO- in physiological pH condition and the detection limit was estimated to be as low as 5.7 nM. Furthermore, its preeminent recognition properties made the successful application for monitoring ClO- in environmental water samples and labeling ClO- in living biological cells.
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