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Published on: June 23, 2020
Surfactant-mediated synthesis of polyhydroxybutyrate (PHB) nanoparticles for sustained drug delivery
Senthilkumar Pachiyappan1, Dawn Shanmuganatham Selvanantham2, Sree Samanvitha Kuppa3
1Department of Chemical Engineering, Sathyabama Institute of Science and Technology, Chennai - 600119, India.
Abstract:
In this study, polyhydroxybutyrate (PHB) nanoparticles were synthesised following nanoprecipitation method having different solvents and surfactant (Tween 80) concentrations. In this study, PHB nanoparticles were encapsulated with curcumin and subjected for sustained curcumin delivery. Both the curcumin loaded and unloaded PHB nanoparticles were characterised using FTIR, SEM, and AFM. Sizes of the particles were found to be between 60 and 300 nm. The drug encapsulation efficiency and in vitro drug release of the nanoparticles were analysed. Antibacterial activity and anticancer activity were also evaluated. The LC50 values of most of the nanoparticles were found to be between 10 and 20 µg/100 µl, anticancer activity of curcumin loaded PHB nanoparticles were further confirmed by AO/PI staining and mitochondrial depolarisation assay.
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