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A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis
Published on: October 24, 2015
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An in vivo Like Micro-Carcinoma Model.
Sandra Camargo1, Yulia Shamis2, Assaf Assis1
1Department of Cell and Developmental Biology, The Hebrew University of Jerusalem, Jerusalem, Israel.
Frontiers in Oncology
|June 14, 2019
Summary
This study introduces a novel micro-system for recreating lung carcinoma in vitro, mimicking in vivo conditions. The system uses acellular micro-scaffolds to support epithelial cells, enabling realistic testing of carcinogenic cell function.
Area of Science:
- Biomedical Engineering
- Cancer Research
- Tissue Engineering
Background:
- Epithelial cells require stromal support in vivo.
- Existing organ-on-a-chip models often lack this crucial stromal component.
- Replicating the in vivo tumor microenvironment is essential for accurate cancer research.
Purpose of the Study:
- To develop a novel micro-system that reconstitutes lung carcinoma in vitro.
- To incorporate epithelial and stromal components in a physiologically relevant manner.
- To provide a platform for testing carcinogenic cell function in a biomimetic environment.
Main Methods:
- Development of acellular micro-scaffolds of microscopic dimensions.
- Seeding of epithelial cells (Calu-3) onto micro-scaffolds.
- Culturing cells in a diffusion-based system for nutrient and gas exchange, avoiding vascularization.
- Analysis of gene expression patterns related to tumors.
Main Results:
- Calu-3 cells formed a well-organized, continuous, polarized, one-layer epithelium.
- The epithelium lined stromal-derived alveolar cavities within the micro-system.
- Gene expression patterns in the micro-system differed from standard monolayer cultures, indicating a more in vivo-like response.
- The model successfully mimicked the natural in vivo situation for epithelial cell support.
Conclusions:
- The novel micro-system effectively reconstitutes a lung carcinoma microenvironment in vitro.
- This platform allows for the study of carcinogenic cell function in a more physiologically relevant context than traditional cell cultures.
- The system's design, based on acellular micro-scaffolds and diffusion, offers a unique approach to organoid modeling.
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