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Related Experiment Video

Updated: Jan 23, 2026

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Controlling DNA Tug-of-War in a Dual Nanopore Device.

Xu Liu¹, Yuning Zhang², Roland Nagel¹

¹Ontera, Inc., Santa Cruz, CA, 95060, USA.

Small (Weinheim an Der Bergstrasse, Germany)

|June 14, 2019

Summary

This summary is machine-generated.

Controlling DNA speed through nanopores is crucial for sequencing. A novel dual-nanopore method uses a "tug-of-war" to significantly slow DNA translocation, enhancing sensing capabilities.

Keywords:

DNA sensing nanopores single molecules tug-of-war two-pore

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Last Updated: Jan 23, 2026

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Published on: October 26, 2016

Area of Science:

Biophysics
Nanotechnology
Molecular Biology

Background:

Accurate DNA sequencing requires precise control over DNA molecule translocation through nanopores.
Current methods often face challenges with translocation speed, limiting sensing performance and feature detection.

Purpose of the Study:

To develop and demonstrate a method for reducing and controlling the speed of DNA translocation through nanopores.
To enhance DNA sensing performance for applications like direct strand sequencing and sequence-specific feature mapping.

Main Methods:

Utilizing two independently controllable nanopores positioned for simultaneous capture of a single DNA molecule.
Implementing active control logic to apply competing voltages, creating a "tug-of-war" effect on the DNA.
Analyzing DNA translocation dynamics and ionic current signals from both pores.

Main Results:

Achieved a "tug-of-war" state in 76.8% of captured molecules, significantly reducing translocation speed.
Demonstrated up to a two-order of magnitude increase in average DNA translocation time.
Quantified translocation slow-down as a function of voltage tuning, consistent with 1D subdiffusive processes.

Conclusions:

The dual-nanopore "tug-of-war" method effectively controls DNA translocation speed and conformation.
Synchronous ionic current measurements from dual pores enable sequential detection of molecular labels.
This approach holds promise for advanced applications such as genome mapping and enzyme-free sequencing.