Thermal Inactivation at High Temperatures and Regeneration of Green Asparagus Peroxidase
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Updated: Jan 23, 2026

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Published on: June 25, 2018
Carmen Rodrigo1, Miguel Rodrigo2, Andrés Alvarruiz3
1Departamento de Medicina Preventiva, Salud Pública, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universitat de València, C/Vicente Andres Estelles s/n, 46100 Burjassot, Valencia.
Abstract:
A spectrophotometric method was developed for determining the peroxidase activity of green asparagus in small samples. The optimum conditions for the analysis in the cuvette were 45 mM of H2O2 36 mM of guaiacol, and pH 7. The method can be used to determine enzyme activity at up to two decimal reductions. A study was performed of the regeneration and inactivation kinetics of the enzyme when heated between 90 and 125°C. Regenerated asparagus peroxidase reached its maximum activity after being stored 6 days at 25°C. The regenerated enzyme followed first-order inactivation kinetics, showing an E = 13.62 kcal/mol and k100°C = 2.07 min-1.
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