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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
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Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus

Jennifer L Endres1, Vijaya Kumar Yajjala1, Paul D Fey1

  • 1Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, NE, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 15, 2019
PubMed
Summary

Transposon mutagenesis creates random bacterial genome mutations for gene function studies. This method generates sequence-defined libraries in Staphylococcus aureus for efficient research.

Keywords:
Random mutagenesisStaphylococcus aureusTransposonbursa aurealis

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