Synthesis of carbon-14-labelled peptides

Carbon-14 (¹⁴ C)-labelled active pharmaceutical ingredients (APIs) and investigational medicinal products (IMPs) are required for phase 0/I to phase III mass balance and micro-dosing clinical trials. In some cases, this may involve the synthesis of ¹⁴ C-labelled peptides, and the analysis can be performed by accelerated mass spectrometry (AMS). The ¹⁴ C-peptide is typically prepared by the solid-phase peptide synthesis (SPPS) approach using custom-made glassware for the key coupling steps. Further modification of the purified ¹⁴ C-peptide can then be performed.