IMPACT OF AG NANOPARTICLES ON MICROORGANISMS, CAUSATIVE AGENTS OF PURULENT-INFLAMMATORY PROCESSES

Area of Science:

• Nanotechnology
• Microbiology
• Materials Science

Background:

• Purulent-inflammatory processes are often caused by bacteria such as Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Candida albicans, and Klebsiella pneumonia.
• Silver nanoparticles (AgNPs) have demonstrated antimicrobial properties, but their efficacy against specific pathogens and their impact on biofilm formation require further investigation.

Purpose of the Study:

• To determine the impact of silver nanoparticles on microorganisms responsible for purulent-inflammatory processes.
• To evaluate the effectiveness of AgNPs in inhibiting bacterial growth and preventing biofilm formation.

Main Methods:

• Testing the antimicrobial activity of AgNPs against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Candida albicans, and Klebsiella pneumonia.
• Assessing the zone of inhibition and bacterial sensitivity at various AgNP concentrations.
• Evaluating the effect of AgNPs on the ability of planktonic cells to form biofilms and on pre-formed biofilms.

Main Results:

• AgNPs exhibited significant growth inhibition zones against Staphylococcus aureus and Streptococcus pyogenes (1-6 mm sample length) and Escherichia coli (5-6 mm sample length).
• Bacterial strains were sensitive to AgNPs at concentrations of 10^4 - 10^6 CFU/ml, but showed resistance at 10^8 CFU/ml and higher.
• AgNP treatment significantly reduced biofilm formation in Candida albicans (3.4) and Klebsiella pneumonia (5.5).
• Disorganization of established biofilms was observed upon AgNP exposure.

Conclusions:

• Silver nanoparticles show promise as antimicrobial agents against key pathogens causing purulent-inflammatory processes.
• AgNP efficacy is concentration-dependent, with higher microbial loads leading to resistance.
• Silver nanoparticles effectively inhibit both initial biofilm formation and disrupt existing biofilms, suggesting dual therapeutic potential.