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Updated: Jan 23, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Published on: January 11, 2017
Protein Microarrays and Liposome: A Method for Studying Lipid-Protein Interactions
Samuel Herianto1, Chien-Sheng Chen2, Heng Zhu3
1High Throughput Biosensing Laboratory, Department of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan City, Taiwan.
Abstract:
Interactions between specific lipids and proteins can provide important information regarding the functions of proteins and lipids. One of the novel and powerful methods to identify lipid-protein interactions is using protein microarrays and liposomes. Liposomes are spherical vesicles that are surrounded by phospholipid bilayers in which the lipid of interest can be incorporated. Thus, liposomes can be used to detect lipid-protein interactions and to analyze interactions between thousands of proteins and a small number of lipids with a single experiment. This chapter presents the methods and procedures for using protein microarray assays and liposome fabrication to analyze protein-lipid interactions. Up-to-date research reports are also reviewed briefly.
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