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Updated: Jan 23, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Bioinformatic Tools and Genome Analysis of Citrus tristeza virus
Ana Belén Ruiz-García1, Rachelle Bester2,3, Antonio Olmos4
1Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, Spain.
Abstract:
High-throughput sequencing (HTS) is a powerful tool employed by plant virologists for the detection of viruses, the characterization of virus genomes and the study of host-pathogen interactions. Virus detection has been an important application of this technology, which has resulted in the discovery of novel viruses or viral strains as well as for the detection of known viruses in a plant sample. Here we describe the entire process that needs to be considered for the genome analysis of Citrus tristeza virus (CTV) by HTS, including the experimental design, sample preparation, nucleic acid purification, HTS library construction, and bioinformatic analysis.
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