Related Experiment Video
Updated: Jan 23, 2026

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
Seven Co-Occurring Mutations in a Patient with Acute Myeloid Leukemia Identified by Next-Generation Sequencing
Background:
Most cases of acute myeloid leukemia (AML) have multiple driver mutations.
Methods:
We report a rare AML case with seven mutations and an aggressive clinical course.
Results:
A 69-year-old woman presented with nausea and vomiting. A bone marrow smear showed increased mye-loblasts, promonocytes, and monocytes. Immunophenotyping identified myeloid and monocytic markers. Fusion transcripts were not detected. Massive parallel sequencing showed seven variants in DNMT3A, FLT3, KRAS, NPM1, PTPN11, and TET2. Five days after beginning chemotherapy, the patient expired.
Conclusions:
These findings may provide insight into the link between multiple mutations and poor prognosis.
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