Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

4.6K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
4.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

2.8K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
2.8K
Vibrating Concrete01:19

Vibrating Concrete

381
Mechanical vibrators are instrumental in compacting newly poured concrete within formwork and around reinforcements. This process is essential to eliminate trapped air pockets and establish a dense concrete mass. One widely used method is vibrating by internal vibrators, often referred to as a poker vibrator or immersion vibrator. It is rapidly inserted through the full depth of the freshly laid concrete and slightly extends into the layer below it (which remains in a plastic state). Consistent...
381
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

1.8K
Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single...
1.8K
Electric Field01:16

Electric Field

12.3K
Consider two point charges, each exerting Coulomb force on the other. It is possible to describe the Coulomb interaction via an intermediate step by defining a new physical quantity called the electric field.
In the new picture, imagine that the first charge sets up an electric field independent of all other charges in the universe. When another charge comes in its vicinity, the second charge experiences an electric force depending on the electric field at that point. The source charge does not...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lysinoalanine crosslinking in the extracellular flagellar hook of Synergistota.

bioRxiv : the preprint server for biology·2026
Same author

Predicting Hunting Trends: The Effect of Pelt Prices, Urbanization and Weather on the Hunting/Trapping of Twelve Small Fur-Bearing Animals in Wisconsin, 1930-2018.

Animals : an open access journal from MDPI·2026
Same author

Bacterial internalins exploit E-cadherin to promote head and neck tumor metastasis and drug resistance.

bioRxiv : the preprint server for biology·2026
Same author

A MinD-like ATPase couples flagellation and cell division in spirochetes.

bioRxiv : the preprint server for biology·2026
Same author

Excited-State Electron-Phonon Coupling in Pristine and Doped Iron Pyrite.

The journal of physical chemistry letters·2026
Same author

Grand Challenges and Opportunities in Stimulated Dynamic and Resonant Catalysis.

ACS catalysis·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026
Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jan 22, 2026

Super-resolution Imaging of Neuronal Dense-core Vesicles
09:30

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

10.1K

Far-Field Super-Resolution Vibrational Spectroscopy.

Christian T Graefe1, David Punihaole1, Celina M Harris1

  • 1Department of Chemistry , University of Minnesota , Minneapolis , Minnesota 55455 , United States.

Analytical Chemistry
|June 29, 2019
PubMed
Summary
This summary is machine-generated.

Researchers are developing label-free super-resolution microscopy using vibrational spectroscopy. This approach avoids fluorescent tags, offering new possibilities for high-resolution imaging in various applications.

More Related Videos

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.3K
Test Samples for Optimizing STORM Super-Resolution Microscopy
16:52

Test Samples for Optimizing STORM Super-Resolution Microscopy

Published on: September 6, 2013

31.6K

Related Experiment Videos

Last Updated: Jan 22, 2026

Super-resolution Imaging of Neuronal Dense-core Vesicles
09:30

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

10.1K
Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.3K
Test Samples for Optimizing STORM Super-Resolution Microscopy
16:52

Test Samples for Optimizing STORM Super-Resolution Microscopy

Published on: September 6, 2013

31.6K

Area of Science:

  • Optics and Photonics
  • Spectroscopy
  • Microscopy

Background:

  • Super-resolution fluorescence microscopy enables nanoscale imaging but requires fluorescent tags.
  • Fluorescent tags can introduce artifacts and limit sample compatibility.
  • Developing label-free alternatives is crucial for broader applications.

Purpose of the Study:

  • To explore vibrational spectroscopies as a basis for label-free super-resolution techniques.
  • To discuss potential sample applications for these novel methods.
  • To outline future directions for enhancing resolution.

Main Methods:

  • Development of super-resolution techniques utilizing vibrational spectroscopies.
  • Analysis of spectral data for nanoscale imaging.
  • Investigation of sample compatibility and potential applications.

Main Results:

  • Demonstration of label-free super-resolution imaging principles.
  • Identification of suitable vibrational spectroscopic modalities.
  • Exploration of diverse sample types amenable to this technique.

Conclusions:

  • Vibrational spectroscopies offer a promising label-free route to super-resolution imaging.
  • This technology has the potential to overcome limitations of fluorescence-based methods.
  • Further advancements could significantly enhance imaging resolution and expand applications.