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Related Concept Videos

2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

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Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
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2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

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Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
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Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

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The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
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NMR Spectroscopy Of Amines01:19

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In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
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2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

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Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
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2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

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Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
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Related Experiment Video

Updated: Jan 22, 2026

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy
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Interleaved spatial/spectral encoding in ultrafast 2D NMR spectroscopy.

Bertrand Plainchont1, Patrick Giraudeau2, Jean-Nicolas Dumez1

  • 1CEISAM, CNRS UMR6230, Université de Nantes, 44300 Nantes, France.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|June 29, 2019
PubMed
Summary
This summary is machine-generated.

Ultrafast 2D NMR (UF2DNMR) limitations are overcome using spatial/spectral pulses (SPSP) for enhanced spectral width and resolution. This method enables detailed analysis of complex molecules like cyclosporin A and metabolite mixtures.

Keywords:
Excitation sidebandsHSQCInterleaved excitationSpatial encodingSpatial/spectral pulsesTOCSYUltrafast 2D NMR

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Physical Chemistry
  • Analytical Chemistry

Background:

  • Ultrafast 2D NMR (UF2DNMR) enables rapid spectral acquisition but faces limitations in spectral width and resolution due to spatial encoding.
  • Traditional linear spatial encoding restricts UF2DNMR performance, necessitating alternative strategies for broader applicability.

Purpose of the Study:

  • To analyze and develop an alternative spatial encoding strategy using spatial/spectral pulses (SPSP) for UF2DNMR.
  • To overcome the spectral width and resolution constraints inherent in traditional UF2DNMR methods.

Main Methods:

  • Numerical simulations were performed to characterize bidimensional SPSP pulses and identify spectral sidebands.
  • An interleaved excitation scheme was developed and experimentally implemented to suppress unwanted harmonic sideband signals.
  • The developed SPSP method was applied to record ultrafast selective TOCSY and HSQC spectra.

Main Results:

  • Numerical simulations revealed sidebands in the spectral dimension of the SPSP excitation profile.
  • The interleaved excitation scheme effectively suppressed signals from harmonic sidebands.
  • Demonstrated applications include ultrafast selective TOCSY for cyclosporin A resonance assignment and ultrafast HSQC for metabolite mixture analysis with optimized spectral width.

Conclusions:

  • The spatial/spectral pulse (SPSP) method offers a viable alternative to linear spatial encoding for UF2DNMR, enhancing spectral width and resolution.
  • The developed interleaved excitation scheme effectively mitigates sideband artifacts, improving spectral quality.
  • This approach significantly expands the potential applications of UF2DNMR in analyzing complex biological and chemical systems.