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Updated: Jan 22, 2026

Exfoliation and Analysis of Large-area, Air-Sensitive Two-Dimensional Materials
Published on: January 5, 2019
Development of enhanced sensitivity protocols on the RapidHIT™ 200 with a view to processing casework material
David Shackleton1, Jenny Pagram2, Nicholas Andrews1
1Key Forensic Services, 4 Penfold Drive, Wymondham, Norfolk NR18 0WZ, UK.
This study enhanced the RapidHIT™ 200 DNA analysis system for crime scene samples. New protocols improved the range of DNA input quantities for reliable forensic profiling.
Area of Science:
- Forensic Science
- Molecular Biology
- Genetics
Background:
- The RapidHIT™ 200 is a sample-to-profile DNA analysis platform.
- Current protocols have limitations in processing crime scene samples with varying DNA quantities.
- The AmpFℓSTR® NGMSElect™ Express PCR Amplification Kit is used for DNA profiling.
Purpose of the Study:
- To evaluate the sensitivity of the 'Run Other' protocol on the RapidHIT™ 200 for crime stain samples.
- To investigate enhancements to the instrument's extraction processes to improve DNA input range.
- To develop new run protocols for broader DNA template processing.
Main Methods:
- Testing the 'Run Other' protocol with crime stain samples and varying DNA input.
- Investigating modifications to the DNA extraction methods within the RapidHIT™ 200.
- Developing and validating new run protocols based on enhanced extraction.
Main Results:
- The initial 'Run Other' protocol showed a limited range of usable DNA input quantities.
- Enhancements to the extraction process significantly improved the range of processable DNA input templates.
- Three new run protocols were developed as a result of these improvements.
Conclusions:
- The optimized extraction methods enhance the RapidHIT™ 200's capability for forensic DNA analysis.
- New protocols expand the utility of the RapidHIT™ 200 for crime scene investigations.
- Improved DNA input range leads to more reliable forensic DNA profiling.
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