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Updated: Jan 22, 2026
08:22
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
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Molecularly Imprinted Polyacrylamide with Fluorescent Nanodiamond for Creatinine Detection
Reim A Almotiri1, Kathryn J Ham1, Vineeth M Vijayan1
1Center for Biophysical Sciences and Engineering (CBSE), Department of Physics, University of Alabama at Birmingham, 421 Campbell Hall, 1300 University Blvd, Birmingham, AL 35294, USA.
Materials (Basel, Switzerland)
|July 3, 2019
Summary
This study developed a novel fluorescent sensor for creatinine detection using imprinted nanodiamonds in a hydrogel. The sensor shows enhanced creatinine affinity and fluorescence changes, paving the way for improved kidney health diagnostics.
Area of Science:
- Biomedical Engineering
- Materials Science
- Analytical Chemistry
Background:
- Creatinine measurement is crucial for kidney health assessment.
- Developing sensitive and specific creatinine detection methods is an ongoing challenge.
- Molecularly imprinted polymers (MIPs) offer selective molecular recognition capabilities.
Purpose of the Study:
- To create a fluorescent sensor for creatinine detection using fluorescent nanodiamonds integrated into a creatinine-imprinted polymer hydrogel.
- To investigate the interaction between creatinine and the imprinted polymer-nanodiamond composite.
- To evaluate the potential of nitrogen-vacancy (NV) centers in nanodiamonds for creatinine sensing.
Main Methods:
- Synthesis of a polyacrylamide hydrogel imprinted with creatinine, incorporating fluorescent nanodiamonds.
- Characterization of nanodiamond distribution and bonding within the hydrogel using Fourier transform infrared spectroscopy and microscopic imaging.
- Measurement of nanodiamond fluorescence quenching and NV center emission ratios in the presence of creatinine.
Main Results:
- The creatinine-imprinted polymer exhibited significantly higher fluorescence quenching compared to the non-imprinted polymer, indicating specific creatinine binding.
- Nanodiamonds formed aggregates within the hydrogel network.
- A 15% decrease in the NV-/NV0 emission ratio was observed for the imprinted polymer upon creatinine addition, attributed to surface charge interactions and changes in diamond's near-surface band structure.
Conclusions:
- The developed fluorescent nanodiamond-imprinted polymer hydrogel demonstrates potential as a sensitive creatinine sensor.
- Nanodiamond fluorescence, particularly NV center emission, can be effectively utilized for creatinine detection.
- Further optimization of nanodiamond dispersion is expected to enhance sensor sensitivity and detection range for improved kidney function diagnostics.
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