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IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol PEG Precipitation
Published on: May 1, 2011
Direct genotyping from whole blood using alkaline polyethylene glycol
Xiaonan Liu1, Chao Zhang1, Kai Hua2
1College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China.
Abstract:
In order to simplify biological sample preparation to meet the demand of rapid genotyping, we improve alkaline polyethylene glycol (APEG) based on Chomczynski's procedure for the PCR-based lateral flow genotyping system, which enables the rapid and efficient direct genotyping from whole blood without DNA purification. The improved APEG has a high tolerance for extreme storage conditions. Testing whole blood with an abnormal hematological index indicates that APEG efficiency would not be influenced by pathological factors. Compared with sequencing, the accuracy of this genotyping system was 100% on testing with 200 clinical samples.
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