Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Steel Manufacturing01:26

Steel Manufacturing

1.4K
Steel manufacturing is a multi-stage process that begins by smelting iron ore into cast iron in a blast furnace. This initial stage involves layering iron ore with coke, a type of fuel, and crushed limestone within the furnace. The coke is ignited with a high volume of air, leading to the creation of carbon monoxide, which acts to reduce the iron ore to pure iron.
During this smelting process, limestone plays a crucial role by forming slag. Slag captures impurities within the molten iron, such...
1.4K
Overview of Exosomes01:36

Overview of Exosomes

3.6K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.6K
Marcia's Theory of Identity Status01:26

Marcia's Theory of Identity Status

1.5K
James Marcia's identity status model provides a framework for understanding how adolescents navigate identity formation through varying degrees of exploration and commitment. Marcia's model builds on Erik Erikson's theories of psychosocial development, focusing specifically on how adolescents reconcile individual aspirations with societal expectations. His model describes identity formation as a dynamic process where adolescents move between different states depending on their level...
1.5K
Manufacture of Concrete Masonry Units01:27

Manufacture of Concrete Masonry Units

390
The process of manufacturing concrete masonry units begins by mixing stiff concrete composed of Portland cement, aggregates, and water. This mixture is then poured into metal molds. To ensure the concrete settles uniformly and to avoid separation of its components, the mixture in the molds is subjected to vibration. Shortly after, the still-wet blocks are removed from the molds and placed on racks.
These wet blocks are then transported for curing, which can occur in one of two environments: a...
390
Formulation and Manufacturing Process: Physical Attributes of Generic Tablets and Capsules01:18

Formulation and Manufacturing Process: Physical Attributes of Generic Tablets and Capsules

312
Bioequivalence in generic drugs, such as tablets and capsules, refers to their pharmaceutical equivalence to the brand-name counterparts. However, for therapeutic equivalence, manufacturers must also consider physical attributes like size, shape, and weight (FDA Guidance for Industry, December 2003). Discrepancies in these aspects could impact patient compliance and cause medication errors. For instance, swallowing difficulties, often experienced with larger tablets or capsules, can lead to...
312
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AI and ATMP: Patients First.

PDA journal of pharmaceutical science and technology·2025
Same author

Improved mRNA affinity chromatography binding capacity and throughput using an oligo-dT immobilized electrospun polymer nanofiber adsorbent.

Journal of chromatography. A·2024
Same author

A Classification of Speakers.

Buffalo medical journal·2023
Same author

Enhancement of Cell-Based Vaccine Manufacturing through Process Intensification.

PDA journal of pharmaceutical science and technology·2021
Same author

Digital biomanufacturing supporting vascularization in 3D bioprinting.

International journal of bioprinting·2020
Same author

Targeting prion propagation using peptide constructs with signal sequence motifs.

Archives of biochemistry and biophysics·2014
Same journal

Indole-based carbohydrazides as potent anti-proliferative leads and tubulin polymerization inhibitors: design, synthesis, cytotoxic evaluation, <i>in silico</i> ADMET and docking studies.

Future medicinal chemistry·2026
Same journal

Recent advances in the development of small molecule STK33 inhibitors.

Future medicinal chemistry·2026
Same journal

Biological evaluation and molecular docking of previously reported pyrazolo-thiazole derivatives as dual α-amylase and α-glucosidase inhibitors.

Future medicinal chemistry·2026
Same journal

Coumarin derivatives as antidiabetic agents: a comprehensive review on mechanistic insights, structure-activity relationships, <i>in silico</i> studies, and challenges.

Future medicinal chemistry·2026
Same journal

Expanding the tetrazole space: medicinal chemistry, biological diversity and structure-activity relationships.

Future medicinal chemistry·2026
Same journal

Thiazolidin-based dual Aurora kinase inhibitors with potent anticancer activity.

Future medicinal chemistry·2026
See all related articles

Related Experiment Video

Updated: Jan 22, 2026

An Innovative Method for Exosome Quantification and Size Measurement
11:38

An Innovative Method for Exosome Quantification and Size Measurement

Published on: January 17, 2015

31.4K

Exosome manufacturing status.

William Whitford1, Peter Guterstam2

  • 1Strategic Solutions Leader, GE Healthcare, BioProcess, Cell Culture, Logan, UT, USA.

Future Medicinal Chemistry
|July 9, 2019
PubMed
Summary
This summary is machine-generated.

Exosomes are key intercellular communicators, but their complex nature and isolation challenges hinder research. Developing scalable platforms is crucial for understanding exosome function and therapeutic applications.

Keywords:
manufacturingproductionexosome

More Related Videos

Isolation and Characterization of RNA-Containing Exosomes
09:43

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

100.4K
Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts
06:27

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

Published on: May 16, 2020

3.9K

Related Experiment Videos

Last Updated: Jan 22, 2026

An Innovative Method for Exosome Quantification and Size Measurement
11:38

An Innovative Method for Exosome Quantification and Size Measurement

Published on: January 17, 2015

31.4K
Isolation and Characterization of RNA-Containing Exosomes
09:43

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

100.4K
Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts
06:27

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

Published on: May 16, 2020

3.9K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Nanotechnology

Background:

  • Exosomes are nanoscale vesicles secreted by mammalian cells, acting as crucial mediators of intercellular communication.
  • They transmit information and materials, playing significant roles in physiological and pathological processes.
  • The full complexity of exosome nature and function remains incompletely understood.

Purpose of the Study:

  • To highlight the significant obstacles in efficient and robust isolation of pure and specific exosomes.
  • To emphasize the need for scalable solutions for exosome separation from other biological entities.
  • To advocate for the development of generic production platforms for exosome manufacturing.

Main Methods:

  • Review of current understanding of exosome biology and intercellular communication.
  • Analysis of existing challenges in exosome isolation and purification techniques.
  • Discussion of the limitations of current manufacturing solutions, including those for Good Manufacturing Practice (GMP).

Main Results:

  • Current understanding of exosome characteristics and their relationship to function is limited.
  • A shortage of scalable solutions for separating specific exosomes from other large entities persists.
  • Existing exosome manufacturing solutions are often adapted from other purposes and may not be optimal.

Conclusions:

  • Efficient isolation of pure and specific exosomes remains a significant challenge.
  • Scalable and generic production platforms are needed for advancing exosome research and application.
  • Further development is required to optimize exosome manufacturing processes, particularly for GMP compliance.