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Related Experiment Video

Updated: Jan 22, 2026

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Published on: April 10, 2016

Light-Sheet Microscopy in Neuroscience.

Elizabeth M C Hillman¹, Venkatakaushik Voleti¹, Wenze Li¹

¹Departments of Biomedical Engineering and Radiology and Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY 10027, USA;

Annual Review of Neuroscience

|July 9, 2019

Summary

This summary is machine-generated.

Light-sheet microscopy illuminates entire tissue planes for faster imaging than point-scanning methods. This advanced technique enables high-speed, in vivo imaging of complex neural tissues and even the mammalian brain.

Keywords:

GCaMP functional imaging light sheet microscopy tissue clearing

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Last Updated: Jan 22, 2026

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

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Published on: February 27, 2014

Area of Science:

Neuroscience
Microscopy
Biotechnology

Background:

Light-sheet microscopy offers advantages over point-scanning techniques like confocal and two-photon microscopy.
Early light-sheet implementations focused on small specimens, but newer versions image freely moving subjects and the in vivo mammalian brain.

Purpose of the Study:

To review the principles and evolution of light-sheet microscopy.
To discuss emerging applications and opportunities in neuroscience imaging.

Main Methods:

Illuminating an entire plane of tissue simultaneously.
Parallelized detection for improved speed and reduced photobleaching.
Volumetric imaging at high speeds.

Main Results:

Light-sheet microscopy achieves higher volumetric imaging speeds compared to point-scanning methods.
Enables imaging of large, cleared, and expanded neural tissues.
Facilitates high-speed, functional imaging in vivo.

Conclusions:

Light-sheet microscopy is a powerful tool for diverse neuroscience applications.
Emerging implementations expand its capabilities for in vivo and large-scale tissue imaging.
Offers significant benefits in speed, photobleaching, and signal-to-noise ratio.