Related Experiment Video
Updated: Jan 22, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
Published on: August 10, 2009
Practical determination of LODP (limit of detection for microarray platform) for the evaluation of microarray
Satoru Adachi1, Hidetoshi Aoki2, Yumi Ueda3
1Special Project Z1, Zeon Corporation, 1-2-1 Yako, Kawasaki-ku, Kawasaki-shi, Kanagawa, 210-9507, Japan.
Abstract:
The performance indicator called limit of detection for microarray platform (LODP) was defined in ISO 16578:2013. The methods to determine practical LODP were explored. In general, + 3 SD of the background is used as the signal strength of limit of detection and criteria for dividing positive and negative results. Since the negative signal had been defined differently for each microarray platform, signals obtained from Non-Probe Spots (NPS) installed on the microarrays were defined as the "background" of microarrays. LODP was determined as the lowest concentration of which the average signal exceeded Avg. + 3 SD of the background (NPS) and the signal was significantly different from those of the lower and higher adjacent concentration points measured with a diluted series of reference materials. For reliable qualitative analysis, the positive results can be defined as signals higher than those corresponding to LODP and negative results as lower signals, without determining limit of detection for all target probes. The use of LODP also enables comparisons of platform performances without checking sequence dependencies, and assists to select reliable and fitting platforms for experimental purposes.
Related Concept Videos
DNA Microarrays
Difference from Background: Limit of Detection
The LOD indicates the presence or absence...
Limiting Reactant
The Number e as a Limit
Central Limit Theorem
The sample size, n, that...
Limits to Natural Selection

