[Scintigraphy with 111In-labeled leukocytes: simplified procedure for labeling]

Area of Science:

• Nuclear Medicine
• Immunology
• Biomedical Imaging

Background:

• Indium-111 (111In) labeled leukocytes are valuable for diagnosing inflammatory conditions.
• Routine clinical use requires simplified, sterile preparation and labeling procedures.
• Maintaining leukocyte viability and function post-labeling is crucial for accurate imaging.

Purpose of the Study:

• To develop and evaluate a simplified, sterile procedure for preparing and labeling leukocytes with 111In oxine sulfate.
• To assess the impact of the labeling procedure on leukocyte viability and chemotaxis.
• To determine the efficiency of 111In chelation and leukocyte labeling.

Main Methods:

• Developed a simplified, sterile protocol for leukocyte separation and labeling using water-soluble oxine sulfate.
• Assessed leukocyte viability and chemotactic function after the labeling process.
• Quantified the chelation rate of 111In with oxine sulfate.
• Determined the labeling efficiency of 111In onto leukocytes.
• Evaluated the quality of in vivo images, specifically looking for erythrocyte-related artifacts.

Main Results:

• The simplified procedure successfully maintained leukocyte viability and chemotaxis.
• Achieved a high chelation rate of 111In with oxine sulfate (93.5%).
• Demonstrated high labeling efficiency of 111In leukocytes (93.8%).
• Obtained clear images without significant blood pool artifacts from residual erythrocytes.
• 111In labeled leukocytes effectively migrated to sites of inflammation.

Conclusions:

• The developed simplified method is suitable for routine clinical use of 111In labeled leukocytes.
• The procedure ensures high labeling efficiency and preserves critical leukocyte functions.
• This technique provides reliable imaging of inflammatory foci with reduced artifacts.