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Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool
Published on: May 25, 2021
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Solid-phase microextraction: An appealing alternative for the determination of endogenous substances - A review
Siming Huang1, Guosheng Chen2, Niru Ye2
1Department of Radiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang Road West, Guangzhou, 510120, China.
Analytica Chimica Acta
|July 17, 2019
Summary
Solid-phase microextraction (SPME) is a key technique for analyzing endogenous substances in complex biological samples. This review covers recent SPME advancements for detecting biomarkers and metabolic pathways in various organisms.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Environmental Science
Background:
- Endogenous substances provide critical biological information, including metabolic pathways and disease biomarkers.
- Complex biological matrices and low analyte concentrations necessitate efficient sample preparation for accurate determination.
- Solid-phase microextraction (SPME) integrates multiple sample preparation steps into a single, efficient process.
Purpose of the Study:
- To review recent developments and applications of SPME for endogenous substance determination over the past five years.
- To highlight advancements in SPME device design and sampling methodologies.
- To discuss the integration of SPME with cutting-edge analytical instrumentation.
Main Methods:
- Review of recent literature on SPME for endogenous substance analysis.
- Analysis of SPME device designs, including sampling configurations and coatings.
- Examination of SPME applications in both in vitro and in vivo sampling scenarios.
- Investigation of SPME coupling with advanced analytical techniques such as GC×GC, AMS, and SERS.
Main Results:
- SPME has demonstrated significant advancements in the determination of endogenous compounds across diverse biological matrices.
- Innovations in SPME device design and coating materials have enhanced extraction efficiency and selectivity.
- Successful coupling of SPME with instruments like GC×GC, AMS, and SERS has expanded its analytical capabilities for complex samples.
Conclusions:
- SPME is a versatile and powerful technique for the analysis of endogenous substances, offering advantages in sample preparation efficiency.
- Continued development in SPME technology and its integration with advanced analytical platforms will further enhance the study of biological systems.
- Addressing current challenges in SPME methods will unlock new opportunities for biomarker discovery and metabolic profiling.
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