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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
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A critical look on CRISPR-based genome editing in plants
Niaz Ahmad1, Mehboob-Ur Rahman1, Zahid Mukhtar1
1Agricultural Biotechnology Division, National Institute for Biotechnology & Genetic Engineering (NIBGE), Faisalabad, Pakistan.
Journal of Cellular Physiology
|July 19, 2019
Summary
Clustered regularly interspaced short palindromic repeats (CRISPR) gene editing offers a powerful tool for crop genetics. However, overlooked limitations like off-targets and environmental risks may hinder its widespread application in agriculture.
Area of Science:
- Agricultural Science
- Genetics
- Biotechnology
Background:
- CRISPR-Cas genome editing, derived from prokaryotic immunity, is a versatile tool for targeted genomic alterations.
- It offers advantages over traditional genetic engineering, including simplicity, cost-effectiveness, and high specificity.
Purpose of the Study:
- To highlight overlooked limitations and challenges associated with CRISPR-based genome editing in crop improvement.
- To critically assess potential risks that may impede the broader application of this technology in agriculture.
Main Methods:
- Review and analysis of existing literature on CRISPR-Cas technology in plant genetics.
- Identification and discussion of under-addressed issues concerning CRISPR applications in crop improvement.
Main Results:
- CRISPR technology faces limitations including off-target mutations, variable mutagenesis efficiency, and reliance on in-vitro regeneration.
- Unaddressed concerns include persistent CRISPR activity, gene flow to wild relatives, reversion of edits, and a scarcity of validated targets.
Conclusions:
- Despite its potential, several critical aspects of CRISPR technology require further investigation and mitigation strategies.
- These overlooked challenges may significantly impact the successful and safe deployment of CRISPR for enhancing crop genetics and agricultural productivity.
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