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Updated: Jan 22, 2026

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
Published on: September 21, 2011
Three new steroidal components from the roots of reineckia carnea
Linfeng Zheng1, Dongdong Zhang1,2, Yuze Li1
1Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, China.
Abstract:
A new coprostanol glycoside, 26-trihydroxy-16, 22-dioxo-3β-[(α-L-rhamnopyranosyl)oxy]-5β-cholestan-1β-yl O-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside (1) (25S)-5β-spirostan-1β,2β,3β, 4β,5β, 6β-hexol (2), a new C-22 steroidal lactone saponin, (20 R)-16β-trihydroxy-3β-[(α-L-rhamnopyranosyl)oxy]-1β-[(O-α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl)oxy]-5β-pregnane-20-oic acid γ-lactone (3) along with two known pregnane glycosides (4 and 5) were obtained from the roots of Reineckia carnea. Their structures were determined by 1 D, 2 D NMR, IR and MS data analysis. In addition, the cytotoxic activities in HT-29, HCT116, H1299 and A549 tumor cells of the isolated compounds (1 - 5) were determined by the MTT method. The results showed that only compound 1 exhibited weak effect against these cells with IC50 values ranging from 63.36 μM to 105.01 μM.
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