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Updated: Jan 21, 2026

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
Published on: September 19, 2022
Macrocyclisation and functionalisation of unprotected peptides via divinyltriazine cysteine stapling
Naomi S Robertson1, Stephen J Walsh, Elaine Fowler
1Department of Chemistry, University of Cambridge, Lensfield Rd, Cambridge, CB2 1EW, UK. spring@ch.cam.ac.uk.
Abstract:
We report a novel divinyltriazine linker for the stapling of two cysteine residues to form macrocyclic peptides from their unprotected linear counterparts. The stapling reaction occurred rapidly under mild conditions on a range of unprotected peptide sequences. The resulting constrained peptides displayed greater stability in a serum stability assay when compared to their linear counterparts.
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