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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
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Complete Genome Sequence of Enterotoxigenic Escherichia coli Myophage LL12

Denish Piya1,2, Lauren Lessor2, Mei Liu3

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|July 27, 2019
PubMed

Insights

The complete genome of virulent myophage LL12 was sequenced. This bacteriophage targets enterotoxigenic Escherichia coli, a cause of traveler's diarrhea, and is used in the prebiotic PreforPro.

Area of Science:

  • Microbiology
  • Genomics
  • Virology

Background:

  • Enterotoxigenic Escherichia coli (ETEC) is a primary cause of traveler's diarrhea.
  • Bacteriophages, viruses that infect bacteria, are being explored for therapeutic applications.
  • Myophage LL12 is a virulent bacteriophage with potential applications.

Purpose of the Study:

  • To present the complete genome sequence of the virulent myophage LL12.
  • To understand the genomic relationship of LL12 to other coliphages.
  • To characterize LL12 for its potential use as a component in prebiotics.

Main Methods:

  • Whole-genome sequencing of myophage LL12.
  • Bioinformatic analysis of the LL12 genome.
  • Comparative genomic analysis with related coliphages.

Main Results:

  • The complete genome sequence of myophage LL12, a 136-kb DNA molecule, was determined.
  • LL12 shares genomic similarities with coliphage V5.
  • LL12 was isolated using enterotoxigenic Escherichia coli as the host.

Conclusions:

  • The genomic data provides a foundation for further research into myophage LL12.
  • LL12's specific targeting of ETEC suggests its potential as a therapeutic agent.
  • LL12 is a viable component for the prebiotic formulation PreforPro.

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