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Building a Simple and Versatile Illumination System for Optogenetic Experiments
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DIY optogenetics: Building, programming, and using the Light Plate Apparatus.

Karl P Gerhardt1, Sebastian M Castillo-Hair1, Jeffrey J Tabor2

  • 1Department of Bioengineering, Rice University, Houston, TX, United States.

Methods in Enzymology
|August 3, 2019
PubMed
Summary
This summary is machine-generated.

We developed an open-source hardware platform, the Light Plate Apparatus (LPA), to make optogenetic systems more accessible. This system uses standard LEDs and an easy-to-use web application for precise light control in molecular biology.

Keywords:
DIYHardwareOpen-sourceOptogeneticsPhotobiologySoftware

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Bioengineering

Background:

  • Optogenetic systems offer precise control over molecular biological processes.
  • Current optogenetic systems often require specialized and expensive hardware, limiting accessibility.
  • There is a need for more accessible tools for optogenetics research.

Purpose of the Study:

  • To describe the development and implementation of an open-source hardware platform for optogenetics.
  • To make optogenetic tools more accessible to researchers.
  • To provide a comprehensive guide for building, programming, and using the Light Plate Apparatus (LPA).

Main Methods:

  • Development of an open-source hardware platform, the Light Plate Apparatus (LPA).
  • Integration of standard LEDs for delivering independent light signals to a 24-well culture plate.
  • Creation of an easy-to-use, open-source web application (Iris) for programming light signals.

Main Results:

  • The Light Plate Apparatus (LPA) was successfully designed and built.
  • The LPA is capable of delivering two independent light signals.
  • The Iris web application allows for the creation of basic to advanced light programs.

Conclusions:

  • The LPA provides an accessible and cost-effective solution for optogenetic control.
  • This open-source platform empowers researchers with precise molecular control.
  • The described system facilitates the adoption of optogenetics in various research settings.