DIY optogenetics: Building, programming, and using the Light Plate Apparatus
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Updated: Jan 21, 2026

Building a Simple and Versatile Illumination System for Optogenetic Experiments
Published on: January 12, 2021
Karl P Gerhardt1, Sebastian M Castillo-Hair1, Jeffrey J Tabor2
1Department of Bioengineering, Rice University, Houston, TX, United States.
Abstract:
Optogenetic systems enable unmatched precision for controlling molecular biological processes but require the use of specialized electrical and optical hardware. In an effort to make working with optogenetic systems more accessible, we recently described an open-source hardware platform called the Light Plate Apparatus (LPA). The LPA is a device capable of delivering two independent light signals from standard LEDs to wells of a 24-well culture plate. Basic to advanced level light programs can be created for the LPA in Iris, an easy to use, open-source web application. In this chapter, we describe each step required to build, program, and use the LPA.
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