Related Experiment Video
Updated: Jan 21, 2026

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding
Published on: September 20, 2024
Integrated physiological and genomic analysis reveals structural variations and expression patterns of candidate
Weibing Zhuang1, Hongxue Wang2, Tianyu Liu2
1Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China. weibingzhuangnj@sina.com.
Abstract:
Colored-leaf plants are increasingly popular and have been attracting more and more attentions. However, the molecular mechanism of leaf coloration in plants has not been fully understood. In this study, a colored-leaf cultivar of Populus deltoides (Caihong poplar, CHP) and green-leaf cultivar of Populus deltoides L2025 were used to explore the mechanism of leaf coloration through physiological and the whole genome resequencing analysis. The content of anthocyanins, total Chl, and carotenoids in the leaves of CHP and L2025 were evaluated. The ratio of anthocyanins to total Chl in CHP was 25.0 times higher than that in L2025; this could be attributed to the red leaf color of CHP. Based on the whole genome resequencing analysis, 951,421 polymorphic SNPs and 221,907 indels were screened between CHP and L2025. Using qRT-PCR analysis, three structural genes (flavonol synthase 1 family protein, UDP-glucose flavonoid 3-O-glucosyltransferase 3' and flavonoid 3-O-galactosyl transferase family protein) and six transcription factors (MYB-related protein Myb4, transcription factor GAMYB, PtrMYB179, transcription factor bHLH53, transcription factor bHLH3, VARICOSE family protein) may be involved in the anthocyanin synthesis pathway, which could be used as candidate genes to explore the molecular regulation mechanism of leaf coloration in Populus deltoids, and could be used in molecular breeding in the future.
More Related Videos
10:40Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
06:57Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives
Published on: August 18, 2023
Related Concept Videos
What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?
Genome Size and the Evolution of New Genes
Chromatin Position Affects Gene Expression
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Cell Specific Gene Expression
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...