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Mechanism-Guided Design of Highly Efficient Protein Secretion and Lipid Conversion for Biomanufacturing and
Shangxian Xie1, Su Sun1, Furong Lin1
1Synthetic and Systems Biology Innovation Hub and Department of Plant Pathology and Microbiology Texas A&M University College Station TX 77843 USA.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|August 6, 2019
Summary
This study engineered bacterial protein secretion for efficient production of ligninolytic laccase, achieving high yields. The research also uncovered mechanisms for lipid production from waste, enabling sustainable biomanufacturing.
Area of Science:
- Biotechnology and Synthetic Biology
- Microbial Engineering
- Biorefining
Background:
- Bacterial protein secretion is crucial for producing valuable biomolecules but faces significant challenges.
- Efficient production of enzymes like laccase is vital for industrial applications, including lignin depolymerization.
Purpose of the Study:
- To optimize the heterologous secretion of ligninolytic laccase in *Rhodococcus opacus* using a proteomics-guided engineering approach.
- To elucidate the mechanisms behind the oleaginous phenotype of *R. opacus* for enhanced lipid production.
- To integrate laccase secretion and lipid production for efficient conversion of biorefinery waste.
Main Methods:
- Proteomics-guided engineering of transcription, translation, secretion, and folding pathways.
- Biochemical analysis of lignin depolymerization.
- Detailed proteomics analysis to understand lipid biosynthesis pathways.
Main Results:
- Achieved a total protein yield of 13.7 g L-1 for secretory laccase.
- Identified a distinct multiunit fatty acid synthase I as key driver for lipid accumulation.
- Demonstrated high-titer lipid production from lignin-enriched waste by integrating laccase secretion and lipid production modules.
Conclusions:
- Proteomics-guided engineering successfully balanced bacterial protein secretion processes, overcoming toxicity and enhancing yield.
- The study revealed mechanisms for oleaginousness in *R. opacus*, guiding efficient lipid conversion from lignocellulosic biomass.
- This integrated approach offers a transformative platform for biomanufacturing and biorefining using waste streams.
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