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Updated: Jul 9, 2026

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

Diol-Induced Lignin Stabilization Facilitates Softwood Saccharification.

Tingjun Chen¹, Jinyuan Cheng², Jing Ren³

¹School of Light Industry and Engineering, South China University of Technology, Guangzhou, China.

|July 8, 2026

Summary

Diol-assisted deep eutectic solvents (DES) modify lignin in softwood, reducing enzyme inhibition. This pretreatment significantly enhances cellulose saccharification for biofuel production.

Keywords:

deep eutectic solvent diols enzymatic saccharification lignin stabilization lignocellulose pretreatment

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Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

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Published on: March 9, 2021

Area of Science:

Biomass Conversion
Biotechnology
Green Chemistry

Background:

Lignocellulose pretreatment aims to improve cellulose accessibility for enzymatic hydrolysis.
Conventional methods often lead to lignin condensation, inhibiting enzyme activity, especially in softwoods.

Purpose of the Study:

To develop diol-assisted deep eutectic solvent (DES) systems for modifying lignin structure.
To alleviate lignin's inhibition on enzymatic saccharification of highly lignified softwood.

Main Methods:

Utilized diol-DES systems for softwood pretreatment.
Characterized modified lignin using Gel Permeation Chromatography (GPC), 2D HSQC NMR, 31P NMR, hydrophobicity measurements, and Langmuir adsorption isotherms.
Assessed enzymatic saccharification efficiency.

Main Results:

Diol-DES treatment resulted in residual lignin with etherified sidechains, reducing nonproductive enzyme adsorption.
Modified lignin exhibited a specific β-O-4 structure, higher aliphatic hydroxyl content, and lower hydrophobicity.
Pinus massoniana cellulose saccharification increased from 31.2% to 90.0%.

Conclusions:

Diol-assisted DES effectively modifies lignin, mitigating its inhibitory effects on cellulase.
This approach significantly enhances enzymatic saccharification of softwood biomass.
The study provides a mechanism for improved biomass conversion through lignin structural modification.