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Updated: Jan 21, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
Published on: March 9, 2017
Programmable Paper-Based Microfluidic Devices for Biomarker Detections
Veasna Soum1, Sooyong Park1, Albertus Ivan Brilian1
1Department of Chemistry, Institute of Biological Interfaces, Sogang University, Seoul 04107, Korea.
Abstract:
Recent advanced paper-based microfluidic devices provide an alternative technology for the detection of biomarkers by using affordable and portable devices for point-of-care testing (POCT). Programmable paper-based microfluidic devices enable a wide range of biomarker detection with high sensitivity and automation for single- and multi-step assays because they provide better control for manipulating fluid samples. In this review, we examine the advances in programmable microfluidics, i.e., paper-based continuous-flow microfluidic (p-CMF) devices and paper-based digital microfluidic (p-DMF) devices, for biomarker detection. First, we discuss the methods used to fabricate these two types of paper-based microfluidic devices and the strategies for programming fluid delivery and for droplet manipulation. Next, we discuss the use of these programmable paper-based devices for the single- and multi-step detection of biomarkers. Finally, we present the current limitations of paper-based microfluidics for biomarker detection and the outlook for their development.
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