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Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
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An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation.

Julie Loh1, Mei-Chun Chuang1, Shan-Shan Lin1

  • 1Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei 10002, Taiwan.

Journal of Cell Science
|August 9, 2019
PubMed
Summary
This summary is machine-generated.

A decrease in plasma membrane tension triggers phosphatidic acid production and macropinocytosis in cells. This newly identified pathway involves phospholipase D2 and is crucial for maintaining cell membrane homeostasis.

Keywords:
EndocytosisMechanical transductionPLD2 nanodomainPhosphatidic acid

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Area of Science:

  • Cell Biology
  • Biophysics
  • Mechanobiology

Background:

  • Endocytosis is vital for cellular functions, responding to biochemical and physical cues.
  • While increased plasma membrane tension effects on endocytosis are known, the impact of decreased tension is less understood.

Purpose of the Study:

  • To investigate how a decrease in plasma membrane tension influences cellular membrane trafficking and endocytic pathways.

Main Methods:

  • Studied myoblasts and myotubes to observe cellular responses to acute decreases in plasma membrane tension.
  • Utilized biochemical assays and microscopy to analyze phosphatidic acid production, F-actin dynamics, and PI(4,5)P2 localization.

Main Results:

  • An acute decrease in plasma membrane tension induced phosphatidic acid production, F-actin, and PI(4,5)P2-enriched membrane ruffling and macropinocytosis.
  • Phosphatidic acid production was dependent on phospholipase D2 activation.
  • This pathway was prominent in myotubes, suggesting a role in muscle cell homeostasis.

Conclusions:

  • Identified a novel mechanotransduction pathway linking decreased plasma membrane tension to macropinocytosis via phosphatidic acid production.
  • This pathway is mediated by phospholipase D2, F-actin, and PI(4,5)P2, contributing to plasma membrane tension homeostasis.