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Updated: Jan 21, 2026

Single Cell Electroporation in vivo within the Intact Developing Brain
Published on: July 11, 2008
Single-Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia
Bianca Brawek1, Olga Garaschuk2
1Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Tübingen, Germany.
Abstract:
Similar to many nonexcitable cells, microglia utilize intracellular Ca2+ signaling for the communication with each other as well as neurons and astrocytes and for triggering a magnitude of their executive functions. However, in vivo measurements of the intracellular Ca2+ dynamics in microglia have been challenging due to technical reasons. Here, we describe an approach utilizing a single-cell electroporation technique to facilitate the study of microglial Ca2+ signaling in the living brain.
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