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Updated: Jan 21, 2026

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
Published on: June 22, 2017
Functional Analysis of Brain-Engrafted Monocytes After Microglia Ablation in Mouse Models
Nicholas H Varvel1, Richard M Ransohoff2,3, Jonas J Neher4,5
1Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA, USA. nicholas.h.varvel@emory.edu.
Abstract:
During insults and disease blood-borne monocytes can invade brain and spinal cord, contributing to the neuroimmune response together with brain-resident microglia. The specific function of brain-infiltrating monocytes has been difficult to ascertain because of shared marker expression and morphology of these two immune cell types. Here we describe our method of repopulating the brain with circulating monocytes after microglia ablation to investigate the physiology of brain-invading monocytes, which engraft under these conditions.
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