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A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
Published on: January 5, 2017
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Alterations in Gene Expression Associated with Head and Neck Squamous Cell Carcinoma Development
Magdalena M Serewko-Auret1, Alison L Dahler1, Louise Smith1
1Epithelial Pathobiology Group, Cancer Biology Program, Centre For Immunology and Cancer Research, University of Queensland, Princess Alexandra Hospital, Queensland, Australia, 4102.
Cancer Genomics & Proteomics
|August 10, 2019
Summary
This study analyzed gene expression in head and neck squamous cell carcinoma (HNSCC) using organotypic raft cultures. Researchers identified 124 significantly altered genes, offering new molecular targets for HNSCC formation.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Utilized organotypic raft cultures to mimic in vivo architecture of normal keratinocytes and head and neck squamous cell carcinoma (HNSCC) cells.
- Investigated gene expression alterations between normal keratinocytes and neoplastic HNSCC cells (SCC-25).
Purpose of the Study:
- To analyze gene expression alterations in HNSCC using gene arrays.
- To identify novel molecular targets for understanding HNSCC development.
Main Methods:
- Organotypic raft cultures were used to generate RNA from normal and neoplastic cells.
- Gene arrays were probed with RNA, and alterations were analyzed using fold differences and 2-tailed t-tests.
- Immunohistochemical staining of patient tumors confirmed gene array data for selected genes.
Main Results:
- Identified 124 significantly altered genes between normal and neoplastic HNSCC cells.
- Observed gene expression alterations across various functional groups, highlighting the complexity of HNSCC gene dysregulation.
Conclusions:
- Discovered numerous novel gene alterations associated with HNSCC.
- Identified significantly altered genes in functional groups including growth control, apoptosis, and detoxification.
- These findings present new targets for investigating the molecular basis of HNSCC formation.
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