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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
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Sesquiterpenoids from Saussurea costus.
Tran Thi Hong Hanh1, Pham Thi Cham1, Nguyen Thi Thuy My2
1Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.
Natural Product Research
|August 13, 2019
Summary
Researchers isolated sixteen sesquiterpenoids from Saussurea costus roots, identifying two new compounds. Three compounds showed significant inhibitory effects on nitric oxide production in cellular assays.
Area of Science:
- Natural Product Chemistry
- Pharmacology
Background:
- Saussurea costus is a plant with a history of traditional medicinal use.
- Sesquiterpenoids are a diverse class of natural products known for various biological activities.
Purpose of the Study:
- To isolate and characterize sesquiterpenoids from Saussurea costus roots.
- To evaluate the inhibitory potential of isolated compounds on lipopolysaccharide (LPS)-induced nitric oxide (NO) production.
Main Methods:
- Isolation of compounds using various chromatographic techniques.
- Structure elucidation employing 1D and 2D Nuclear Magnetic Resonance (NMR) and High-Resolution Quadrupole Time-of-Flight Mass Spectrometry (HR-QTOF-MS).
- Assessment of anti-inflammatory activity by measuring NO production in RAW264.7 cells stimulated with LPS.
Main Results:
- Sixteen sesquiterpenoids were isolated, including two novel compounds, saussucostusosides A and B.
- Costunolide, 3β-[4-hydroxymethacryloyloxy]-8α-hydroxycostunolide, and 11β,13-dihydrozaluzanin C demonstrated potent inhibition of NO production.
- The IC50 values for these compounds were determined to be 7.08±0.34 µM, 2.40±0.06 µM, and 5.55±0.24 µM, respectively.
Conclusions:
- The study successfully identified and characterized sesquiterpenoids from Saussurea costus.
- Specific sesquiterpenoids from Saussurea costus possess significant anti-inflammatory properties by inhibiting NO production.
- These findings suggest potential therapeutic applications for Saussurea costus-derived compounds in managing inflammatory conditions.
