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An isoelectric focusing study in herpes simplex virus encephalitis
L M Grimaldi1, R P Roos, R Manservigi
1Department of Neurology, University of Chicago, IL 60637.
Annals of Neurology
|August 1, 1988
Summary
An early diagnostic test for herpes simplex virus type 1 encephalitis (HSVE) detected anti-gB antibodies in serum and cerebrospinal fluid (CSF). This test shows promise for timely HSVE diagnosis and treatment.
Area of Science:
- Neurovirology
- Immunology
- Diagnostic Development
Background:
- Herpes simplex virus type 1 encephalitis (HSVE) is a severe neurological condition requiring early diagnosis.
- Current diagnostic methods can be invasive or lack sensitivity for early detection.
Purpose of the Study:
- To develop and evaluate a reliable early diagnostic test for HSVE.
- To detect specific antibodies against HSV-1 glycoprotein B (gB) in patient samples.
Main Methods:
- Isoelectric focusing (IEF) and an IEF-overlay technique were employed.
- Radiolabeled HSV glycoprotein B (gB) was used to detect anti-gB antibodies.
- Serum and cerebrospinal fluid (CSF) samples from HSVE patients were analyzed.
Main Results:
- Anti-gB antibodies were detected in all tested serum (7/7) and most CSF (10/12) samples from HSVE patients.
- Antibodies were found in CSF as early as days 3-5 of the disease.
- Evidence suggested both hematogenous and local antibody production in the central nervous system.
Conclusions:
- The IEF-overlay technique is a promising tool for early HSVE diagnosis.
- Detection of anti-gB antibodies in CSF can aid in differentiating HSVE from other neurological conditions.
- This method may facilitate prompt treatment initiation for HSVE patients.