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Progenitor/Stem Cell Delivery by Suprarenal Aorta Route in Acute Kidney Injury
Érika B Rangel1,2,3, Samirah A Gomes1,4, Rosemeire Kanashiro-Takeuchi1,5
1Interdisciplinary Stem Cell Institute, Leonard M Miller School of Medicine, University of Miami, USA.
Cell Transplantation
|August 15, 2019
Summary
Intra-aortic stem cell delivery promotes kidney repair in rats after injury. This method shows promise for future human clinical trials in regenerative medicine.
Area of Science:
- Regenerative Medicine
- Nephrology
- Stem Cell Biology
Background:
- Progenitor/stem cell therapy is crucial for kidney disease treatment.
- Cell delivery route impacts kidney repair efficiency in acute and chronic injury models.
- Optimizing cell delivery is essential for successful regenerative strategies.
Purpose of the Study:
- To describe and evaluate a protocol for intra-aortic progenitor/stem cell injection in rats.
- To assess the efficacy of this delivery method in models of acute kidney injury and podocyte damage.
- To determine engraftment rates and recovery outcomes following intra-aortic stem cell transplantation.
Main Methods:
- Developed an intra-aortic injection protocol in rats following ischemia-reperfusion injury or puromycin aminonucleoside (PAN)-induced nephropathy.
- Administered green fluorescent protein (GFP)-labeled c-Kit+ progenitor/stem cells or GFP-mesenchymal stem cells (2 x 10^6) via suprarenal aorta.
- Utilized vascular clips on the abdominal aorta and renal pedicles to control blood flow during injection.
Main Results:
- Achieved approximately 10% engraftment rates with c-Kit+ progenitor/stem cells at day 8 post-ischemia-reperfusion injury, accelerating kidney recovery.
- Observed similar engraftment rates after PAN-induced podocyte damage at day 21.
- Demonstrated a high success rate (100%) for injection and significant recovery (∼85%) within a week post-injection.
Conclusions:
- Intra-aortic progenitor/stem cell injection is an effective method for promoting kidney repair in rodent models.
- This technique facilitates significant cell engraftment and functional recovery in acute kidney injury and podocyte damage.
- Findings support the translation of intra-arterial cell delivery strategies to human clinical trials using endovascular catheters.
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