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P21-Associated ncRNA DNA Damage-Activated Expression in Bladder Cancer

Feraydoon Abdolmaleki1, Soudeh Ghafouri-Fard1, Mohammad Taheri2

  • 1Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Klinicka Onkologie : Casopis Ceske a Slovenske Onkologicke Spolecnosti
|August 21, 2019
PubMed

Insights

This study investigated PANDA long non-coding RNA in bladder cancer, finding no significant difference in expression between tumor and normal tissues. PANDA is unlikely to be involved in bladder cancer development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are crucial in carcinogenesis.
  • Dysregulation of lncRNA expression contributes to cancer pathogenesis.
  • PANDA ncRNA, located near CDKN1A, is involved in DNA damage response, cellular senescence, and proliferation.

Purpose of the Study:

  • To evaluate the expression of PANDA ncRNA in bladder cancer tissues.
  • To compare PANDA expression in tumor tissues, adjacent non-cancerous tissues (ANCT), and normal bladder tissues.
  • To determine the association of PANDA expression with clinical and demographic data.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) was used to assess PANDA lncRNA expression.
  • Expression levels were analyzed in bladder cancer tissues, ANCT, and normal bladder tissues.
  • Statistical analysis was performed to compare expression levels and correlate them with clinical parameters.

Main Results:

  • No significant difference in PANDA expression was found between bladder tumors and ANCT (expression ratio = 1.75, p = 0.11).
  • No significant difference in PANDA expression was observed between tumors and normal tissues (expression ratio = 2.72, p = 0.57).
  • PANDA expression levels did not correlate with tumor grade, recurrence, or risk factors like smoking or opium use.

Conclusions:

  • PANDA long non-coding RNA does not appear to be involved in the pathogenesis of bladder cancer.
  • Further research on other lncRNAs may aid in identifying biomarkers for bladder cancer.
Keywords:
DNA damage-activatedP21-associated ncRNAPANDARNAlong non-codingurinary bladder neoplasms

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